منابع مشابه
Separation techniques: Chromatography
Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques ba...
متن کاملSeparation Science - Chromatography Unit
Before examining chromatographic separations, it is useful to consider the separation process in a liquid-liquid extraction. Certain features of this process closely parallel aspects of chromatographic separations. The basic procedure for performing a liquid-liquid extraction is to take two immiscible phases, one of which is usually water and the other of which is usually an organic solvent. Th...
متن کاملPaper Chromatography: Separation and
The example of column chromatography (Figure 1) demonstrates the typical features found in this analytical technique. The diagram shows an experiment where a two-component mixture is subjected to column chromatography. The column is packed with a solid material called the stationary phase. A liquid solvent or eluting solution is poured into the column and completely wets the solid packing mater...
متن کاملHigh-pressure liquid chromatography: separation
The high-pressure liquid chromatographic separation of ail of the known metabolites of vitamin D2 and vitamin D3 found in biological fluids has been achieved. This technique has been successfully applied to the analysis of vitamin D mixtures, purification of vitamin D metabolites, and identification of radioactive peaks. Some theoretical bases for the observed resolutions are suggested. Supplem...
متن کاملSeparation of hydroxyanthraquinones by chromatography
Было изучено хроматографическое поведение гидроксиантрахинонов. Хорошее разделение было достигнуто при использовании предложенных новых хроматографических систем: бумажная хроматография смесью уксусной кислоты и воды на простой бумаге или бумаге импрегнированной оливковым мас лом, тонкослойная хроматография на целлюлозе импрегнированной диметилформамидом и на силикагеле без или с импрегнацией ...
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ژورنال
عنوان ژورنال: Northern Clinics of Istanbul
سال: 2016
ISSN: 2148-4902
DOI: 10.14744/nci.2016.32757